LOCUS       ENGASE-pGEc2            7455 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(6933..7062)
                     /note="3'-ITR"
     misc_feature    complement(6631..6845)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    6083..6624
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    5262..5284
                     /note="attB2"
     misc_feature    6062..6067
                     /note="BstB1 site"
     misc_feature    5285..5980
                     /note="Super GFP"
     misc_feature    5981..6031
                     /note="AviTag"
     misc_feature    6032..6055
                     /note="His Tag"
     misc_feature    3011..5239
                     /note="OPTIMIZED ENGASE coding region"
     PCR_frame       3011..5261
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    5240..5261
                     /note="TEV"
     vector          join(5270..7455,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..5269
                     /source="ENGASE pDONR"
                     /type="Entry Clone"
     source          1..7455
                     /dnas_title="ENGASE-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGGAAGCCG CTGCTGTGAC AGTGACAAGA TCCGCCACAA GAAGAAGGCG
     3061 GAGACAGCTG CAGGGACTGG CTGCTCCTGA AGCCGGAACA CAGGAGGAAC AGGAAGATCA
     3121 GGAACCCAGA CCTCGGCGGA GAAGGCCTGG CAGATCTATC AAGGACGAGG AAGAGGAAAC
     3181 CGTCTTTCGC GAGGTGGTGT CCTTCAGCCC TGATCCTCTG CCTGTGCGGT ACTACGACAA
     3241 GGATACCACC AAGCCCATCA GCTTCTACCT GAGCAGCCTG GAAGAACTGC TGGCCTGGAA
     3301 ACCTCGGCTG GAAGATGGCT TCAATGTGGC CCTGGAACCT CTGGCATGTA GACAGCCTCC
     3361 ACTGTCCAGC CAGAGGCCTA GAACACTGCT GTGCCACGAT ATGATGGGCG GCTACCTGGA
     3421 CGACAGATTC ATCCAGGGAA GCGTGGTGCA GACCCCCTAC GCCTTTTACC ACTGGCAGTG
     3481 CATCGACGTG TTCGTGTACT TCTCCCACCA CACCGTGACA ATCCCTCCCG TGGGATGGAC
     3541 AAATACCGCC CACAGACATG GCGTGTGCGT GCTGGGAACC TTCATCACCG AATGGAATGA
     3601 GGGCGGCAGA CTGTGCGAAG CTTTTCTGGC AGGCGACGAG AGATCTTACC AGGCCGTGGC
     3661 TGATAGACTG GTCCAGATCA CCCAGTTCTT TAGATTCGAC GGCTGGCTGA TCAACATCGA
     3721 GAACAGCCTG TCTCTGGCCG CCGTGGGCAA TATGCCTCCA TTCCTGAGAT ACCTGACCAC
     3781 CCAGCTGCAT AGACAGGTGC CAGGTGGACT GGTGCTGTGG TACGATTCTG TCGTGCAGTC
     3841 TGGCCAGCTG AAGTGGCAGG ATGAGCTGAA CCAGCACAAC CGGGTGTTCT TCGATAGCTG
     3901 CGACGGCTTC TTCACCAACT ACAATTGGAG AGAGGAACAC CTGGAACGGA TGCTGGGACA
     3961 GGCCGGGGAG AGAAGGGCTG ATGTGTATGT GGGAGTGGAT GTGTTCGCCA GGGGAAACGT
     4021 CGTCGGAGGC AGATTCGATA CCGACAAGTC TCTGGAACTG ATCCGGAAGC ACGGCTTTTC
     4081 CGTGGCTCTG TTTGCTCCTG GCTGGGTGTA CGAGTGTCTG GAAAAGAAGG ACTTCTTTCA
     4141 GAATCAGGAT AAGTTCTGGG GCAGGCTGGA ACGCTACCTG CCTACACACA GCATCTGCTC
     4201 CCTGCCCTTC GTGACCAGCT TTTGTCTGGG CATGGGCGCT AGAAGAGTGT GCTACGGCCA
     4261 GGAGGAGGCA GTCGGACCTT GGTATCATCT GAGCGCCCAG GAAATCCAGC CTCTGTTCGG
     4321 AGAACACAGA CTGGGGGGAG ATGGTAGAGG ATGGGTCCGA ACACACTGTT GCCTCGAAGA
     4381 TGCTTGGCAT GGCGGTTCTA GTCTGCTCGT GCGGGGAGTG ATTCCCCCCG AAGTGGGAAA
     4441 TGTGGCTGTC AGACTGTTTA GTCTGCAGGC CCCCGTGCCC CCTAAGATCT ACCTCAGCAT
     4501 GGTGTACAAG CTGGAAGGCC CCACAGATGT GACTGTGGCT CTCGAACTGA CAACTGGCGA
     4561 CGCCGGAAGC TGTCACATCG GAGGAATTAG CGTGCTGAAC GCCGAGACAA GCAGCAGACA
     4621 TAGCCTGAGG CCACTGAGAG TGCCACCTAC CAAACTGGCT AGATGGGTCG GAAGATGTGG
     4681 CAGGCAGCTC TCTGGTGGAT GGGTGCAGCA CTGTTACGAG GTGTCCCTGA GAGGATGCCT
     4741 GCTGCTGGAT CTCCTCGTGT GCTTTAGCAG ACCTCCCGGC AGCAGAGAAG AAGAGAGCTT
     4801 CACCTGTCGG CTGGGCGAGA TTCAGGTGGT CGATGCTGCA AGTCTGCTGG CTCCACTGCC
     4861 TCAGGTGCAG GCTGTGACCA TCTCTCACAT CAGATGGCAG CCTAGCGCCT CTGAGAGAGA
     4921 GGGACCTCCT GCTCTGCTCC AGCTGAGCTG TACCCTGCAC TGGTCTTTTC TGCTGAGCCA
     4981 GGTCCGATGC TTTCGGATCC ACTGTTGGGG CGGCATGTCT GATGATAGCC CCGGCAGAGA
     5041 ACTGCCAAGA CCCGAGATGC CTATGTTTCT GGGCCTGGCC TTTGCCACCC AGTACAGGAT
     5101 TGTGGATCTG CTGGTGGAAG CTGCCGGACC CGGACAGGAT AGAAGGATGG AATTTCTGGT
     5161 CGAGCCCGTG CCTAAAGAGG GCTTTAGAGT CCCACAGGCC GAATGGGGTA GAGCTGTGCT
     5221 GCTGTATTCT GCCCCTGCTg aaaacttgta ctttcaaggc tCCAGCTTTC TTGTACAAAG
     5281 TGGTATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG TGGAACTGGA
     5341 TGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGAGAG GGCGAAGGCG ACGCCACAAA
     5401 TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CTTGGCCTAC
     5461 CCTCGTGACC ACACTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG ACCATATGAA
     5521 GCGGCACGAC TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAGGAAC GGACCATCAG
     5581 CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG GCGACACCCT
     5641 CGTGAACCGG ATCGAGCTGA AGGGCATCGA TTTCAAAGAG GACGGCAACA TCCTGGGCCA
     5701 CAAGCTGGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA AGCAGAAGAA
     5761 CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG TGCAGCTGGC
     5821 CGACCACTAC CAGCAGAACA CCCCCATCGG AGATGGCCCC GTGCTGCTGC CCGACAACCA
     5881 CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG ACCACATGGT
     5941 GCTGCTGGAA TTCGTGACCG CCGCTGGCAT CACCCACGGC ATGAGCGGCC TGAACGACAT
     6001 CTTCGAGGCC CAGAAAATCG AGTGGCACGA GCACCACCAT CATCACCATC ACCACTGATG
     6061 ATTCGAAAAA TAATAAGGAT CCAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC
     6121 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT
     6181 GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT
     6241 GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT
     6301 GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG
     6361 GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG
     6421 CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT
     6481 GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT
     6541 CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC
     6601 TCTGCGGCCT CTTCCGCGTC TTCGAGATCT GCCTCGACTG TGCCTTCTAG TTGCCAGCCA
     6661 TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC
     6721 CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG
     6781 GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT
     6841 GGGGACTCGA GTTAAGGGCG AATTGCCGAT TAGGATCTTC CTAGAGCATG GCTACGTAGA
     6901 TAAGTAGCAT GGCGGGTTAA TCATTAACTA CAAGGAACCC CTAGTGATGG AGTTGGCCAC
     6961 TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA CCAAAGGTCG CCCGACGCCC
     7021 GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC AGCCTTAATT AAGAGCTCAT
     7081 GGCGCGCCTA GGCCTTGACG GCCTTCCGCC AATTCGCCCT ATAGTGAGTC GTATTACGTC
     7141 GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT
     7201 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC
     7261 CGAAACGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGAGCGC CCTGTAGCGG
     7321 CCACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC
     7381 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA
     7441 ACGCTTACAA TTTAG
//

